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The Screening Recombinant Cell

Screening for recombinants promega,screening for recombinants transfer spin column to a collection tube. centrifuge. overnight culture centrifuge. clear lysate. transfer lysate. bind dna. wash, removing solution by centrifugation or vacuum. elute plasmid dna. remove culture media. resuspend cells. lyse cells. neutralize. 10.0 6.0 4.0 2.0 1.5

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